Part:BBa_K4624608

FapR B3+B4+B5 Reverse primer for GoldenBraid 2.0 cloning.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage

This part was designed for the addition of 3' appropriate overhangs to FapR (BBa_K4624003) compatible with the 3' of the B3+B4+B5 position of the GoldenBraid 2.0 cloning grammar. This part is meant to be used with FapR B3+B4(+B5) Forward primer (BBa_K4624606).

Figure 1: Gel electrophoresis of the PCR products for the (4) FapR B3+B4+B5 part with primers BBa_K4624606 and BBa_K4624608. Expected bands (bp): Lane 4: 602, L= 1kb plus DNA ladder.

The successful addition of the appropriate 3' and 5' overhangs was confirmed upon the succesful insertion of the part into the universal part domesticator plasmid pUPD2, which is compatible only with domesticated parts, such as the PCR product.